Recombinant Macaca mulatta Microtubule-associated protein tau (MAPT) (Active)

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中文名称：

猕猴MAPT重组蛋白
货号：

CSB-MP013481MOW
规格：

￥1908
促销：
图片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Macaca mulatta MAPT at 2 μg/ml can bind anti-MAPT recombinant antibody (CSB-RA013481A1HU),the EC₅₀ is 2.464-3.979 ng/mL. Biological Activity Assay
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纯度：

Greater than 95% as determined by SDS-PAGE.
内毒素：

Less than 1.0 EU/ug as determined by LAL method.
生物活性：

Measured by its binding ability in a functional ELISA. Immobilized Macaca mulatta MAPT at 2 μg/mL can bind anti-MAPT recombinant antibody (CSB-RA013481A1HU),the EC₅₀ is 2.464-3.979 ng/mL.
基因名：

MAPT
Uniprot No.：

P57786
别名：

(Neurofibrillary tangle protein) (Paired helical filament-tau) (PHF-tau)
分子结构：
种属：

Macaca mulatta (Rhesus macaque)
蛋白长度：

Full Length of Mature Protein
来源：

Mammalian cell
分子量：

50.6 kDa
表达区域：

2-459aa
氨基酸序列

AEPRQEFDVMEDHAGTYGLGDRKDQEGYTMLQDQEGDTDAGLKESPLQTPAEDGSEELGSETSDAKSTPTAEDVTAPLVDERAPGEQAAAQPHMEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSATKQVQRKPPPAEPTSERGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPAREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL
蛋白标签：

N-terminal 10xHis-tagged
产品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
产品描述：

Recombinant Macaca mulatta microtubule-associated protein tau (MAPT) is an active protein produced in mammalian cell lines. The gene encoding the 2-459aa of the Macaca mulatta MAPT was fused with the 10xHis-tag at the N-terminus and then cloned into the plasmid vector, finally transfected into the mammalian cells for subsequent expression. This recombinant MAPT protein was purified and reached over 95% in purity measured by SDS-PAGE. Its endotoxin is less than 1.0 EU/ug determined by the LAL method. Importantly, the activity of this protein has been validated in a functional ELISA in which it can bind the anti-MAPT recombinant antibody, with the EC₅₀ of 2.464-3.979 ng/mL.

MAPT plays an important role in the occurrence and development of neuropathy-related diseases. The accumulation of hyperphosphorylated MAPT in neurons will cause neurofibrillary degeneration, which is associated with a variety of neurodegenerative diseases such as Alzheimer's disease (AD) and PD. Non-human primates (NHP) show high homology with humans in physiological function and drug metabolism. In particular, their brains are most similar to humans in structure and function. Therefore, scientists are hoping to be able to model human disease by building NHP models, especially in neuroscience. MAPT protein from Macaca mulatta goes beyond the limits of rodent studies.
Datasheet & COA：

Please contact us to get it.

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平均分:

5.0分 - 1 个评价

实验应用：抗原

产品评价：这次买的是冻干粉，收到产品后我们按照说明书复溶，很好溶解，之后进行SDS质控，纯度高于标准，免疫小鼠后，包被抗原，通过ELISA筛选到多株亲和力较好的阳性克隆，腹水纯化后获得抗体特异性好，灵敏度高。产品整体性能不错，发货快，介绍很详细，工作人员也很专业，非常有耐心，真心推荐！

By 肖老师

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功能：

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
基因功能参考文献：
1. age-related increase in cAMP-dependent protein kinase (PKA) phosphorylation of tau at serine 214 (pS214-tau) in monkey dorsolateral prefrontal association cortex specifically targets spine synapses and the Ca(2+)-storing spine apparatus. PMID: 24707050
亚细胞定位：

Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell projection, axon. Cell projection, dendrite. Secreted.
组织特异性：

Expressed in neurons.
数据库链接：

KEGG: mcc:574327

STRING: 9544.ENSMMUP00000001080

UniGene: Mmu.3731

Recombinant Macaca mulatta Microtubule-associated protein tau (MAPT) (Active)

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