多克隆抗体定制服务

多克隆抗体存在于免疫动物的血清中，可通过直接分离血清获得。由于具有可识别多个表位、可引起凝集反应、沉淀反应等优点，被广泛应用于免疫学诊断领域。比如经常用到的Western blotting实验中的酶标二抗和ELISA实验中的包被抗体。而且制备费用也较单克隆抗体低。

华美生物自主建立抗体研发生产平台，可为您提供可靠的多克隆抗体定制服务，并有多种免疫原和多种宿主物种可供选择。您可以选择自己提供抗原或者我们帮您设计并制备抗原，我们将提供给您满足您实验需求的多克隆抗体产品。

服务流程：

服务内容及周期：

免疫原	服务内容	交付内容	服务周期
多肽	抗原制备动物免疫血清滴度检测亲和纯化或Protein A/G纯化抗原 WB 验证	提供抗原QC报告； ELISA滴度效价保证1:64000；抗原WB检测结果保证阳性；提供1mL免疫前血清，2mL抗血清，5-10mg经protein A/G或亲和纯化的抗体；提供SDS-PAGE检测结果，抗体纯度保证90%。	12-14 周
重组蛋白			14-16 周
天然蛋白			12-14 周

服务实例：

ELISA
Antigen coating concentration 2 ug/ml
Antiserum 1:2000 is more than diluted
Secondary：Goat polyclonal to rabbit IgG at 1/50000 dilution

Western Blot
Positive WB detected in：K562 whole cell lysate,HL-60 whole cell lysate,Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate,Rat liver tissue, Mouse brain tissue, Mouse lung tissue
All lanes：a antibody at 2ug/ ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 64 KDa
Observed band size: 64 KDa

Western Blot
WT: Wild-type 293 cells
KO: Knockout 293 cells

IHC image of A antibody diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with A antibody at 1:400,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).