Rat Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ ELISA KIT

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产品详情

产品描述：

This Rat TNFsR2 ELISA Kit was designed for the quantitative measurement of Rat TNFsR2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.
别名：

Tnfrsf1b ELISA Kit; Tnfr2 ELISA Kit; Tumor necrosis factor receptor superfamily member 1B ELISA Kit; Tumor necrosis factor receptor 2 ELISA Kit; TNF-R2 ELISA Kit; Tumor necrosis factor receptor type II ELISA Kit; TNF-RII ELISA Kit; TNFR-II ELISA Kit; p75 ELISA Kit; p80 TNF-alpha receptor ELISA Kit; CD antigen CD120b ELISA Kit
缩写：

TNFsR-
Uniprot No.：

Q80WY6
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TNFsR-II in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	87-94
1:2	Average %	97
	Range %	92-101
1:4	Average %	90
	Range %	87-98
1:8	Average %	92
	Range %	85-95

回收率：

The recovery of rat TNFsR-II spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-96
EDTA plasma (n=4)	98	95-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2.
基因功能参考文献：
1. results suggested that PTE may promote BMSC proliferation and osteogenic differentiation via a mechanism associated with the regulation of Let-7f-5p and the TNFR2/PI3K/AKT signaling pathway. PMID: 29975930
2. Curcumin decreased TNFR2 and increased TRAF2 to decrease the apoptotic pathway in ureteral obstruction rats. PMID: 26732833
3. The IL-17RD.TNFR2 complex in activation of NF-kappaB may explain the role of TNFR2 in inflammatory diseases including nephritis. PMID: 25378394
4. the microglial TNF-alpha p55 pathway played a more important role than the TNF-alpha p75 pathway in the pathogenesis of peripheral nerve injury pain. PMID: 22652595
5. study of TNF-alpha and TnfrR2 protein synthesis and expression patterns in sciatic nerve of controls and rats under systemic challenge with lipopolysaccharide. PMID: 17873366
6. In human biopsies and in rat kidney allografts on day 6 with acute allograft rejection, significantly elevated expression of TNFR2 was observed. PMID: 19298452
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亚细胞定位：

Membrane; Single-pass type I membrane protein.
数据库链接：

KEGG: rno:156767

STRING: 10116.ENSRNOP00000022478

UniGene: Rn.83633