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  4. Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA kit

Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA kit

文献引用 (1)
  • 中文名称：
    大鼠5` -核苷酸酶，胞膜(NT5E/CD73)酶联免疫试剂盒
  • 货号：
    CSB-E14100r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 别名：
    Nt5e ELISA kit; Nt5 ELISA kit; Nte5'-nucleotidase ELISA kit; 5'-NT ELISA kit; EC 3.1.3.5 ELISA kit; Ecto-5'-nucleotidase ELISA kit; CD antigen CD73 ELISA kit
  • 缩写：
    NT5E/CD73
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates, cell culture supernates
  • 检测范围：
    0.625 mIU/mL-40 mIU/mL
  • 灵敏度：
    0.156 mIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CD73 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %87-96
    1:2Average %87
    Range %82-95
    1:4Average %90
    Range %85-97
    1:8Average %93
    Range %86-101
  • 回收率：
    The recovery of rat CD73 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-101
    EDTA plasma (n=4)9186-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    402.504 2.416 2.460 2.358
    201.933 1.824 1.879 1.777
    101.386 1.283 1.335 1.233
    50.814 0.749 0.782 0.680
    2.50.424 0.397 0.411 0.309
    1.250.228 0.213 0.221 0.119
    0.6250.154 0.145 0.150 0.048
    00.103 0.101 0.102 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • 亚细胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    5'-nucleotidase family
  • 数据库链接：

    STRING: 10116.ENSRNOP00000015057

    UniGene: Rn.40132

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