Pig interleukin 2,IL-2 ELISA Kit

产品详情

文献引用 (6) 说明书下载

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Pig IL2 ELISA Kit was designed for the quantitative measurement of Pig IL2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .
别名：

IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit
缩写：

IL2
Uniprot No.：

P26891
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	80-99
1:2	Average %	86
	Range %	82-91
1:4	Average %	91
	Range %	86-97
1:8	Average %	94
	Range %	88-98

回收率：

The recovery of pig IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-102
EDTA plasma (n=4)	96	91-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
基因功能参考文献：
1. We observed that besides TCR stimulation Tregs require IL-2 for activation. PMID: 21530576
2. a parameter of early mRNA expression during intestinal ischemia reperfusion injury PMID: 15050130
3. The isolation and characterization of interleukin 2 from Tibetan swine are reported. PMID: 16195934
亚细胞定位：

Secreted.
蛋白家族：

IL-2 family
数据库链接：

KEGG: ssc:396868

STRING: 9823.ENSSSCP00000009697

UniGene: Ssc.16224