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  4. Mouse Lipopolysaccharides(LPS) ELISA Kit

Mouse Lipopolysaccharides(LPS) ELISA Kit

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  • 中文名称：
    小鼠脂多糖/内毒素（LPS）酶联免疫试剂盒
  • 货号：
    CSB-E13066m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：
        LPS（脂多糖）是细菌细胞壁中的一种脂类分子，在炎症和感染等过程中起重要作用。LPS可与人体免疫系统中的受体结合，从而诱导炎症反应和免疫细胞的激活。
        华美生物所提供的Mouse Lipopolysaccharides(LPS) ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测鼠血清、血浆、组织匀浆样本中的LPS，其灵敏度为0.039 ng/mL，检测范围为0.156 ng/mL-10 ng/mL。
     
  • 缩写：
    LPS
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 80-92  
    1:2 Average % 98  
    Range % 91-105  
    1:4 Average % 102  
    Range % 92-110  
    1:8 Average % 96  
    Range % 86-98  
  • 回收率：
    The recovery of mouse LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 89-98  
    EDTA plasma (n=4) 94 90-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.424 2.522 2.473 2.406  
    5 2.051 1.955 2.003 1.936  
    2.5 1.345 1.255 1.300 1.233  
    1.25 0.679 0.655 0.667 0.600  
    0.625 0.461 0.444 0.453 0.386  
    0.312 0.257 0.239 0.248 0.181  
    0.156 0.171 0.165 0.168 0.101  
    0 0.069 0.065 0.067 　  
  • 本试剂盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Mouse LPS antibody.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled LPS antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle) ---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubating.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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问答及客户评论

■ 常见问题解答
Q:

Hi, i have some questions. i am interested about CSB-E13066m elisa kit.its sample types showed to serum, plasme etc. i want serum ascites type

A:
Thanks for your inquiry.
Serum and serum ascites are 2 different sample types.Serum is more normal,and you can refer the information in the published papers at the bottom of the product page.

If you want to detect serum ascites,we suggest you do pre-testing first.Pls let me know if you have any further questions. Thank you.
Q:

When using Mouse Lipopolysaccharides(LPS) ELISA Kit, can I finally translate the results (ng/ml) into the EU/ml?
what is the EU/ml of the standard provided?

A:
Thanks for your inquiry.The the results (ng/ml) can not be translated into the EU/ml results as there is no reduction formula between them.
This kit is supplied with 2 vial of frozen dry standard and the concentration is 10 ng/mL after dissolution;Pls let me know if you have any further questions. Thank you.
Q:

I am curious what product you would recommend to detect mouse lipopolysaccharides with very small volumes (less than 10 uL) 

A:
Thanks for your inquiry. The amount is too small, if detected, false negatives may exist.Pls consider it carefully.Pls let me know if you have any further questions. Thank you.
Q:

Please provide the following information regarding Mouse Lipopolysaccharides, LPS ELISA Kit (CSB-E13066m)
Can customer use diluent buffer to dilute their plasma samples?

A:
Thanks for your inquiry.
CSB-E13066m
The reagent system of this kit is very special, and dilution is not needed if you test serum or plasma samples.
If you confirm that dilution is needed after the pretest, we suggest you use sample diluent. About pretest pls refer to: https://www.cusabio.com/m-226.html
Pls let me know if you have any further questions. Thank you.
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靶点详情

  • 最新研究进展：
    脂多糖（LPS）是细菌细胞壁的一种成分，在感染和炎症中发挥重要作用。最新研究表明，LPS除了其已知的免疫活性外，还可能具有其他生物学功能，例如参与代谢调节和神经保护等。此外，研究人员正在探索LPS与肠道微生物组和健康之间的关系，以及利用LPS模型研究多种疾病的发生机制和治疗策略。

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